新型微波天线热凝大鼠坐骨神经的实验研究

新型微波天线热凝大鼠坐骨神经的实验研究

内容简介：目的 观察新型微波天线热凝SD大鼠坐骨神经后,神经形态和功能的长期连续变化.方法 75只SD大鼠随机分成A、B、C组,分别以10 W×6 s、20 W×6 s、30 W×6 s的功率时间组合热凝其坐骨神经,于热凝后0,2,7,30,60 d进行电生理检测和超微结构观察.于热凝后7,30,60 d检测其静态坐骨神经功能指数.结果 B、C两组热凝后0,2,7,30 d时的静态坐骨神经功能指数、神经传导速度和复合动作电位最大幅值都有下降,与热凝前比较差异有统计学意义(P＜0.05),但B组于60 d后恢复接近热凝前.而C组未见恢复.A组热凝后超微结构改变轻微,B组热凝后髓鞘疏松,进而崩解变性坏死.但在60 d后神经纤维再生修复.C组热凝后髓鞘崩解断裂或凝固坏死、轴突坏死、基底膜消失,60 d后大量胶原纤维生成阻碍神经纤维再生.结论 微波热凝神经能导致神经传导功能的下降甚至消失,通过热凝毁损神经而缓解疼痛,微波热凝神经止痛可作为一种新的疼痛治疗手段.

Abstract：Objective To observe the sequential ultrastroctural and electrophysiological changes in the sciatic nerve coagulated by a newly-designed microwave antenna. Methods A total of 75 Sprague-Dawley rats were randomly divided into groups A,B and C and irradiated with microwaves at 10,20 or 30 Watts,for 6 seconds to coagulate the left sciatic nerve.Electrophysiological effects and sequential uhrastructural changes were observed on the 0th,2nd,7th,30th and 60th days after coagulation.A static sciatic index was calculated based on measurements of the footprint on the 7th,30thand 60th days after coagulation.Results On the Oth,2nd,7th and 30th days after cpagulation,the static sciatic index,the nerve conduction velocity and the amplitude of the action potentials in groups B and C had decreased significantly compared with those before coagulation.On the 60th day after coagulation.significant recovery was observed in groups A and B,but not in group C.Only mild alteration in uhrastructure was found,and only in group A.The prominent changes in uhrastructure in group B included broken Schwann cell membranes and myelin disintegration.There were severe injuries in group C,including myelin disintegration,cell deformity,coagulative necrosis,axon necrosis,basement membrane necrosis and demyelination.The structure of the sciatic nerve in group B had partially recovered after 60 days,but group C showed no recovery at all. Conclusion Microwave coagulation of a nerve can block its conduction.and even destroy the nerve.Percutaneous microwave coagulation is clinically feasible and call be an alternative treatment for pain.

作者：何金霞, 张俊德, 朱伟良, 李虹义, 张积仁,

关键词：微波, 坐骨神经, 神经电生理,