内周天线CP43、CP47中β-Car到Chla分子间的能量传递

内周天线CP43、CP47中β-Car到Chla分子间的能量传递

内容简介：采用超快荧光光谱动力学对从菠菜中分离纯化的内周天线CP43、CP47进行研究，获取了它们的动力学三维荧光谱，CP43的荧光光谱范围为640～780nm，最大峰位于680nm处，在该峰值处的荧光寿命约为3.54ns；CP47的荧光光谱范围为630～775nm，最大峰位于691nm处，在该峰值处的荧光寿命约为3.22ns，在CP43和CP47中，Chla分子发射荧光的效率分别约为38.3%和40.6%.依据分子的退激发途径，我们分析认为在CP43、CP47中β-Car→Chla分子的能量传递速率常量分别为9.06×1011s－1，1.3×1012s－1；能量传递效率分别为47.5%、66.5%；并估计β-Car分子与Chla分子外周之间的距离分别为0.110nm、0.085nm.

Abstract：CP43 and CP47，purified from spinach，were studied by using ultrafast fluorescence spectroscopy.And their dynamic fluorescence spectra were gotten.For CP47，the fluorescence spectrum range is 640～780nm and peak at～681nm with fluorescence lifetime 3.54ns.For CP47，the fluorescence spectrum range is 630～775nm and peak at～691nm with fluorescence lifetime 3.22ns.Chla emission efficiencies in CP43 and CP47 are about 38.3% and 40.6% respectively.According to molecule′s deexcitation ways，we analyzed energy transfer of β-Car→Chla in CP43 and CP47 and determined that energy transfer rate constants are 9.06×1011s－1 and 1.3×1012s－1，energy transfer efficiencies 47.5% and 66.5% respectively.Also the distances between the surfaces of β-Car and Chla in CP43 and CP47 were evaluated to be about 0.110nm and 0.085nm.

作者：贺俊芳, 王水才, 张舒, 贺锋涛, 任兆玉, 单际修, 李良壁, 匡廷云,

关键词：能量传递, 超快荧光光谱, PSⅡ, CP43, CP47, 内周天线, β-Car, Chla,